Disperse—a software system for design of selector probes for exon resequencing applications
نویسندگان
چکیده
SUMMARY Selector probes enable the amplification of many selected regions of the genome in multiplex. Disperse is a software pipeline that automates the procedure of designing selector probes for exon resequencing applications. AVAILABILITY Software and documentation is available at http://bioinformatics.org/disperse
منابع مشابه
PieceMaker: selection of DNA fragments for selector-guided multiplex amplification
We describe PieceMaker, a software tool for the design of applications of selector probes-oligonucleotide probes that direct circularization of target nucleic acid molecules. Such probes can be combined in parallel to circularize a selection of fragments from restriction digested total genomic DNA. These fragments can then be amplified in a single PCR using a common primer pair, yielding substr...
متن کاملPartitioning Transcript Variation in Drosophila: Abundance, Isoforms, and Alleles
Multilevel analysis of transcription is facilitated by a new array design that includes modules for assessment of differential expression, isoform usage, and allelic imbalance in Drosophila. The ∼2.5 million feature chip incorporates a large number of controls, and it contains 18,769 3' expression probe sets and 61,919 exon probe sets with probe sequences from Drosophila melanogaster and 60,118...
متن کاملTargeted resequencing of candidate genes using selector probes
Targeted genome enrichment is a powerful tool for making use of the massive throughput of novel DNA-sequencing instruments. We herein present a simple and scalable protocol for multiplex amplification of target regions based on the Selector technique. The updated version exhibits improved coverage and compatibility with next-generation-sequencing (NGS) library-construction procedures for shotgu...
متن کاملA model of base-call resolution on broad-spectrum pathogen detection resequencing DNA microarrays
Oligonucleotide microarrays offer the potential to efficiently test for multiple organisms, an excellent feature for surveillance applications. Among these, resequencing microarrays are of particular interest, as they possess additional unique capabilities to track pathogens' genetic variations and perform detailed discrimination of closely related organisms. However, this potential can only be...
متن کاملReSequencer : an automated primer picking application for gene resequencing efforts
Summary: ReSequencer is a stream-lined, cross-platform, standalone application that is capable of retrieving the DNA sequence of a gene’s exons and then designing primers to sequence each exon. This application remotely accesses Ensembl to search retrieve the DNA sequence for any known transcript for a gene. Once the sequence for each exon is retrieved, ReSequencer runs a client-side binary of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Bioinformatics
دوره 25 شماره
صفحات -
تاریخ انتشار 2009